A summary of the article: Development and Validation of a Novel LC-MS/MS Opioid Confirmation Assay: Evaluation of beta -glucuronidase Enzymes and Sample Cleanup Methods
He, S. Yang, A., Wu, H.B., Lynch, L. K. (2016) Journal of Analytical Toxicology 40:5, 323-329.
With the rise in use and prescription of opioids for pain management over the past few years, an increase in the need to monitor opioid usage has emerged. Typically, testing for opioids and their metabolites in urine is done through immunoassay screening and GC-MS analysis, which involves laborious sample preparation and cleanup. This study sought to develop and validate a more efficient and accurate method for detecting opioids in urine via β-glucuronidase hydrolysis, protein precipitation sample cleanup, and LC-MS/MS assay. Four different β-glucuronidases and three different protein precipitation plates were compared to determine the best enzyme and plate to use for opioid confirmation.
Reference: He, S. Yang, Alan, H.B.Wu, Lynch, L. Kara (2016) Development and Validation of a Novel LC-MS/MS Opioid Confirmation Assay: Evaluation of beta-glucuronidase Enzymes and Sample Cleanup Methods. Journal of Analytical Toxicology 40:5, 323-329