Antidepressants are regularly prescribed to treat depression as well as a variety of other disorders and diseases, and they can also be abused. Because of the widespread usage of antidepressants, patient monitoring is necessary for improved clinical healthcare. Tricyclic antidepressants are heavily glucuronidated when metabolized by the human body, resulting in the need for a hydrolysis step during sample preparation. A new and efficient method for the quantitation of eight cyclic antidepressants in human urine was developed and validated with the use of the genetically modified β-glucuronidase IMCSzyme® coupled with fast LC-MS/MS for quick and accurate analysis.
The results of the tetracyclic and tricyclic antidepressants validation proved that the newly developed method is accurate. Since tricyclic antidepressants are heavily conjugated, IMCSzyme® was selected as the enzyme of choice for hydrolysis because of its quick and efficient performance.
Summary by IMCS from the technical poster “ LC-MS/MS Method for the Determination of Tricyclic and Tetracyclic Antidepressants in Human Urine” presented by Amber F. Awad – Dominion Diagnostics at ASMS 2016