Poster Session: Wednesday, January 25 – #58C
Poster Title: “Dispersive-pipette Extraction for Automated Enrichment of IGF-1 from Serum”
Accurate and robust quantification of insulin-like growth factor 1 (IGF-1) from serum using mass spectrometry (MS) is challenging due to its high complexity and dynamic range. A low cost, high throughput enrichment method for such low abundant proteins from the serum is required for target proteins prior to MS analysis. We report a high-throughput sample preparation method using reverse phase, micro-solid phase extraction enrichment on Hamilton Nimbus. Over 71% recovery of IGF-1 from serum for 96 samples is demonstrated in less than 30 minutes.
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