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Poster Session: #56B Wednesday, January 25
Poster Title: “Conversion of drug metabolites due to contaminants in commercial β-glucuronidase products”
Small molecule drugs undergo phase II glucuronidation and sulfation in the liver to be excreted invarious biological fluids. β-glucuronidase and sulfatase are used to hydrolyze the metabolites, enabling more sensitive and accurate quantitation of the metabolites by mass spectrometry (MS). Any contamination in these enzymes affects not only the effectiveness of the enzyme but also alters the analytes which can skew the outcome. We demonstrate esterase contamination in various commercial β-glucuronidase enzymes using a fluorescent substrate. Contaminated enzymes show the conversion of 6-monoacetylmorphine (6-MAM), a specific metabolite of heroin, to morphine, skewing the readout to false negative for heroin usage.experiment, we focused on how to reduce the time and amount of materials needed to efficiently hydrolyze high concentrations of four glucuronidated opiates and opioids: codeine, morphine, oxymorphone, and hydromorphone.