Phosphorylation events are key signal transduction mechanisms within the eukaryotic cells and further understanding such signaling pathways can elucidate crucial molecular signatures of various human diseases. The global deep profiling of phosphospeptide has been successfully optimized in the procedures of enrichment, mass spectrometry, and data analysis. However, phosphopeptide enrichment is highly labor-intensive and a low-throughput process that leads to poor reproducibility. IMCS R&D team developed a high throughput method using TiO2 dispersive pipette extraction, IMCStips to improve efficiency for phosphopeptide enrichment. In comparison to conventional solid phase extraction technologies, the patented dispersive pipette extraction process uses loose resin that mixes during aspiration and dispense steps.