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21Jun 2017

Automated Peptide Desalting Using Dispersive Pipette Extraction

Desalting peptides is an essential procedure for improving LC-MS/MS analysis (1-3). Removing salts utilizes solid phase extraction (SPE), which has relied on cartridge formats. These formats have been used for routine sample preparation and the microspin columns have been developed to miniaturize and speed up the desalting processes. However, these procedures are not readily adapted for automated liquid handlers. Here, we present automated, dispersive pipette extraction technology in IMCStips for robust and high-throughput proteomics analysis. Several different resins were compared in spin column versus dispersive pipette (4). Specific peptides were monitored for optimal loading and recoveries for both SPE formats (spin vs tip), and total proteome analysis was assessed on cell lysates to determine total protein IDs for both extraction processes.

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