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20Jul 2017
Jul 20, 2017

Comparison of Novel β-Glucuronidases for Process Improvement in Urine Toxicology Workflows

IMCSzyme was featured in the technical poster presentation ” Comparison of Novel β-Glucuronidases for Process Improvement in Urine Toxicology Workflows” at the American Society of Mass Spectrometry Conference -ASMS 2017. Below is a summary of the poster presentation and a technical note available for download.

Overview: 

Detection of the drug compounds via LC-MS/MS. New recombinant glucuronidases have been introduced which are much cleaner and more efficient, avoiding many issues that are present with crude enzyme solutions. This study compares the substrate specificity and conversion efficiency of several different purified β-glucuronidases, as well as assessing side reactions from acetylase and sulfatase activity.

The substrate specificity varied greatly among the tested enzymes. Hydrolysis efficiency of amitriptyline, codeine, and morphine in particular was wide-ranging, with IMCSzyme hydrolyzing the highest percentage of these substrates. No significant acetylase or sulfatase activity was observed for any of the enzymes.

Download Technical Note

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